A technique for detecting Treponema pallidum through the use of membrane filters and immunofluorescence staining.
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منابع مشابه
A technique for detecting Treponema pallidum through the use of membrane filters and immunofluorescence staining.
Danielsson (1965) has recently described the use of non-fluorescent membrane filters and immunofluorescence staining for the identification of certain bacteria in water. The principal advantages of the procedure were that it was rapid; that it could separate and identify a small number of organisms from a relatively large volume of fluid; and that it allowed a quantitative estimate of the conce...
متن کاملSpecific immunofluorescence staining of Treponema pallidum in smears and tissues.
To date, tissue sections prepared from Formalin-fixed tissues have not been successfully stained with Treponema pallidum subspecies-specific antibody in a direct fluorescent-antibody assay. While current methods stain T. pallidum, they do not distinguish T. pallidum from other spirochetes such as Borrelia burgdorferi (E. F. Hunter, P. W. Greer, B. L. Swisher, A. R. Simons, C. E. Farshy, J. A. C...
متن کاملTreponema pallidum surface immunofluorescence assay for serologic diagnosis of syphilis.
A surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and Treponema pallidum immobilization (TPI) assays for detecting serum antibodies to T. pallidum in patients with syphilis, in disease controls, and in healthy subjects. SIFA and WB were 99% sensi...
متن کاملAssessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies.
A rapid test based on an immunochromatography assay - Determine Syphilis TP (Abbott Lab.) for detecting specific antibodies to Treponema pallidum was evaluated against serum samples from patients with clinical, epidemiological and serological diagnosis of syphilis, patients with sexually transmitted disease other than syphilis, and individuals with negative serology for syphilis. The Determine ...
متن کاملThe Morphology and Staining Characteristics of the Treponema pallidum. Review of the Literature and Description of a New Technique for Staining the Organism in Tissues *
In 1905, sixty-eight years after Donne" (cf. Kolmer," Hoffmann," Ingraham5) described a regularly spiraled micro-organism in the exudates of primary syphilitic lesions of the genitalia, Schaudinn and Hoffmann""' recognized a pale, similarly spiraled organism in primary lesions and inguinal lymph nodes of syphilitic patients. Donne, who published his findings in 1837, believed that he had discov...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1969
ISSN: 1368-4973
DOI: 10.1136/sti.45.4.305